Heavy left wing
Wanted to answer a few questions that you posted
Michael, it is a Trio AP, and the servo is mounted in the wing.
Sam, It is the manual spring.
The Trio servo has a clutch which completely releases the gear train when it isn't engaged, not sure how the servo could impact roll trim when it isn't 'on'. I suspect something has changed with the aileron trim spring setup. The installer may have reconnected something differently or possibly stretched a spring.
Is the servo in the wing or cabin? Cabin installations are much more sensitive to mis-rigging than having the servo in the wing.
More info would be useful......roll is induced with or without the autopilot engaged? Can you trim out the roll with aileron trim? Is roll constant at all airspeeds, etc, etc. Did the A&P remove a flap??